Professional Recombinant Subcloning
Applied Viromics offers a wide range of custom molecular biology and subcloning services. Our molecular engineering capabilities enable us to build highly customized constructs optimized for viral gene delivery and downstream expressions.
Our Molecular Biology Services Include:
Commercial & Custom Cloning
Cloning your Gene of Interest (GOI) into any commercial vector, custom destination vector, or Applied Viromics' proprietary in-house viral shuttle plasmids.
Fusion Tagging
N-terminal or C-terminal fusion tag additions (e.g. Flag, HA, Myc, His, GST, GFP) to allow easy identification or purification of your expression product.
RNAi & Gene Silencing
Design, synthesis, and cloning of siRNA or shRNA expression cassettes under U6 or H1 promoters for efficient knockdowns.
Multigene Expression Engineering
Designing and constructing complex multigene vectors using IRES elements or ribosomal skip 2A peptides (P2A, T2A, E2A, F2A) for stoichiometric multi-protein expression.
Cre-loxP Systems
Conditional expression system construction incorporating Cre-loxP elements for tissue-specific or inducible expression control.
Custom Recombinant Design
General vector repair, site-directed mutagenesis, promoter exchanges, and unique chimera vector fabrications.
Starting Materials
- Complete sequence or restriction map of the GOI
- Complete sequence or map of the destination vector
- 2 μg to 10 μg of starting plasmid DNA material
Deliverables
- 2 μg to 4 μg of purified, completed recombinant plasmid
- Restriction digestion gel analysis verifying cloning accuracy
- Electronic plasmid vector map and sequence files
Pricing & Timeline
The price and timeline depend on cloning complexity (GC-rich content, repetitive sequences). Most subcloning projects are completed within **2 weeks**.
Request Cloning Quote